Plasmid

Part:BBa_K2908679

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-17)


s(ESR1)p-miR141spe-EGFP

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR141 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR141-sponge(BBa_K2908555) to bind different concentrations of miR141 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein. T--CSU_CHINA--2908679.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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